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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N489

Name: N489

ABRC stock number: CS489

Description: Ler was transformed with the pCLO461 construct (wild type Ac in GUS); the Ac element was cloned into a binary vector containing a 35S-GUS fusion; Ac was excised from the plasmid pJJ4361 on a Sal1-Sst1 fragment and ligated into the Sal1-Xho1 site of pSLJ732 which contains the 35S-GUS fusion; these transformed lines have been used to study cell patterning and clonal relationships in the roots of Arabidopsis, by following the pattern of GUS expression as a result of Ac excision (Scheres et al, 1994; Dolan et al, 1994).

Donation Date: 1999-03-01

Donated by:  John Innes Centre Caroline Dean
John Innes Centre Clare Lister
John Innes Centre Emily Lawson

Donor Number: C3770

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Seed type: Ds tagged

Mutagen: transposon insertion

Background: Landsberg erecta (Ler)